๐Ÿ”ฌ Soft-Agar Overlay

Means of plating that involves two layers of class="bap-term" title=_blank>media, one consisting of hard or bottom agar that serves as a base and the other consisting of soft or top agar which is poured over the base.

by Stephen T. Abedon Ph.D. (abedon.1@osu.edu)

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About this entry: Soft-Agar Overlay is one of a growing set of phage biology and phage therapy terms defined here. For more phage terms, see Abedon, S.T. (2025). Phage Therapy Annotated Glossary. Preprints.org. 10.20944/preprints202508.0347.v1

phage.org/terms/soft_agar_overlay.html  ·  Abedon’s Books  ·  DOI: 10.5281/zenodo.20173633

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Soft-Agar Overlay: Means of plating that involves two layers of class="bap-term" title=_blank>media, one consisting of hard or bottom agar that serves as a base and the other consisting of soft or top agar which is poured over the base.

Discussion

<p>For additional discussion and references, see Kropinski et al. (2009).

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How to cite this page

Abedon, S.T. (2026). Phage Terms. https://terms.phage.org  ·  10.5281/zenodo.20173633

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Soft-Agar Overlay — terms.phage.org — phage.org — Stephen T. Abedon — Version 2026.05.14